When we do Spirit Rescues for human spirits, when they choose to incarnate again will the Protocol healing be implemented for them in perpetuity, just as for clients in the physical who receive a Lightworker Healing Protocol session?

Indeed, the Protocol request is applied to all clients, to have repeated healing done in perpetuity. In effect, your session includes many clients of all kinds: people, dark spirits, extraterrestrials, extraterrestrial spirits, and animals, so there is consideration by divine realm to have the broadest possible interpretation here in order to spread the love. This is the beauty of making sweeping open-ended requests, as it can provide a wonderful launching pad for a tremendous healing outreach, as long as the healing outreach has within it enough details as to what it must consist of to provide appropriate benefits. Having a broad coverage makes the very most of the effort in asking, so there is no limit on the size of the target and its components. The limit is only in the requester and their energetic reach. So it matters not whether you do work for a person in the physical or a person in spirit form, trapped in the lower astral plane. The same request applies, that all will receive healing, and this will be done in perpetuity whenever they are back in a new incarnation.